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dc.contributor.advisorHu, James C
dc.creatorKnapp, Gwendowlyn Sue
dc.date.accessioned2010-01-15T00:11:22Z
dc.date.accessioned2010-01-16T00:47:56Z
dc.date.available2010-01-15T00:11:22Z
dc.date.available2010-01-16T00:47:56Z
dc.date.created2008-05
dc.date.issued2009-05-15
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-2655
dc.description.abstractProtein-protein interactions regulate and drive biological processes and understanding the assembly of these interactions is important. The LysR-Type Transcriptional Regulators (LTTRs) are a large family of transcriptional regulators found in prokaryotes. I have used the LTTRs as a model for protein specificity. In order to understand a residue’s contribution to oligomerization, alanine-scanning mutagenesis was used to probe the contribution of residues identified from in silico analysis of two proteins: OxyR and CynR. The contribution of the residues to oligomerization was characterized using lcI repressor fusions. In OxyR, seven residues were identified as hot spots. Moreover, these hot spots are not especially conserved. The interaction surface of OxyR was mapped onto a multiple sequence alignment of the LTTR family. This mapping identified putative contacts in the CynR regulatory domain dimer interface. Combined with the in vivo testing, three residues were identified as hot spots. The residues identified in OxyR and CynR do not overlap. To investigate the assembly of the LTTRs I used a negative-dominance assay with lcI repressor fusions. Taken together, I show that the LTTRs in E. coli K-12 are mostly specific in their interactions.en
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.subjectprotein-protein interactionsen
dc.subjectLysR-Type Transcriptional Regulatorsen
dc.titleOligomerization of the lysr-type transcriptional regulators in Escherichia Colien
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiochemistry and Biophysicsen
thesis.degree.disciplineBiochemistryen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberBenedik, Michael
dc.contributor.committeeMemberReinhart, Gregory D.
dc.contributor.committeeMemberYoung, Ryland
dc.type.genreElectronic Dissertationen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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