WAvelength selection for optimal determination of glucose concentration in biological media

Abstract

Current research in the large scale production of cell culture samples will require the use of a dedicated monitoring system capable of determining concentrations of the important growth controlling factors within cell culture media. Current methods are limited to bench top laboratory use and require the large amounts of extra resources of space, materials and time. Other researchers have shown the ability to determine glucose concentration in solution through spectroscopic techniques but have required large regions of the electromagnetic spectrum to be accurate. This research presents the results of using wavelength selection routines to decrease the required spectral region to nearly 10% of the original while achieving similar or in some instance better predictive accuracy. Using concentrations of 0-27.3mM glucose in solution, predictive errors of 0.3 mM were achieved.