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dc.creatorRittenhouse, Kevin Dee
dc.date.accessioned2012-06-07T22:46:33Z
dc.date.available2012-06-07T22:46:33Z
dc.date.created1996
dc.date.issued1996
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-1996-THESIS-R58
dc.descriptionDue to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.en
dc.descriptionIncludes bibliographical references.en
dc.descriptionIssued also on microfiche from Lange Micrographics.en
dc.description.abstractPortions of the mitochondrial DNA control region were used to determine the species of animal hair used in a 17th century merchant ship's hull sheathing for the purpose of deterring wood boring worms (Toredo navalis). DNA was extracted from the hair shafts after the samples were cleaned from the pitch in which they were embedded. The extracted DNA was amplified via the polymerase chain reaction and subsequently sequenced using the dideoxy chain termination method. The sequence was used in a BLAST search (Basic Local Alignment Search Tool provided by the National Center for Biotechnology Information @ http://www.ncbi.nlm.nih.gov/BLAST/) of Genbank to identify animals that had similar sequence content of the same region. The matching sequences were used in a phylogenetic study to determine the species of the hair samples. The results of the phylogenetic study showed that the hair was consistent with that of northern European cattle and led to the conclusion the ship was most likely sheathed in a northern European port. The mitochondrial DNA control region also was used in a study to determine if dog DNA could be recovered from the clothes of a young child allegedly attacked by dogs. The child's parents previously had been convicted of murder and sentenced to life in prison. They were granted habeas corpus relief and released from prison when new evidence suggested that the child may have been attacked by dogs. The Raines County (Texas) District Attorney's Office asked for help in finding evidence that might have allowed for a second indictment on the parents. The child's clothes were obtained from the Raines County District Attorney for genetic analysis. The biological samples recovered from the child's pants were treated as above with the exception that I compared the resultant sequence with known sequences of canids. The clothes also were used in an Indirect Enzyme Linked Immuno-Sorbant Assay (ELISA) to search for dog serum proteins. The results of both studies showed the biological evidence from the clothes was consistent with samples of known dogs, wolves and coyotes.en
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.subjectgeneticsen
dc.subjectMajor geneticsen
dc.titleMitochondrial DNA control region sequences used for identification of species in two forensic science case studies: the Monte Cristi shipwreck and a homicide caseen
dc.typeThesisen
thesis.degree.disciplinegeneticsen
thesis.degree.nameM.S.en
thesis.degree.levelMastersen
dc.type.genrethesisen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen


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