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Conception rates after artificial insemination as affected by heparin binding protein-B5 in frozen semen
Abstract
An experiment was conducted to determine the effect of heparin binding proteins (HBP-B5) on fertilizing capacity of frozen-thawed bovine semen. Frozen semen was obtained from Brahman (n=8) and Brangus (n=6) bulls and evaluated for the presence of HBP-B5 (21, 24, and 31 kDa forms) on the sperm membranes. Bulls were classified into one of three fertility groups, High (H), Medium (M), or Low (L), according to their BBP-B5 profile. Non-lactating, multiparous Angus or Hereford cows (n--108) were assigned for mating with semen from a Brahman sire in the H, M, or L BBP-B5 classification. Lactating, multiparous Angus cows (n=178) were assigned for mating with semen from Brangus sires in the H, M, or L BBP-B5 classification. Cows were observed twice daily for estrus during the 45-d Al breeding season. Pregnancy was determined by palpation per rectum 60 d after the end of the breeding period. Main effects of HBP-B5 classification on pregnancy rate were determined by chi square analysis. Effects of treatment on services per conception were determined by GLM procedures. Pregnancy rates for non-lactating cows inseminated with semen from L, M, or H BBP-B5 sires (P>.10) were 86, 79, and 90%, respectively. Non-lactating cows mated to H HBP-B5 sires required fewer (P<.05) services per conception (1.23 + .09) than cows mated to M HBP-B5 sires (1.52+ .20). Services per conception in non-lactating cows mated to L HBP-B5 sires (1.33+ .07) did not differ from either H or M HBP-B5 sires. Pregnancy rates for lactating cows mated to L, M, or H HBP-B5 sires (P>.10) were 76, 73, and 72%, respectively. Services per conception in lactating cows did not differ among L, M, or H HBP-B5 sires (1.51 + .07, 1.56 +.10, and 1.52 + .07, respectively). Probabilities to become pregnant were not significantly increased due to bull fertility as projected by HBP-B5 profile. Increased cow body condition improved probabilities of becoming pregnant in lactating cows and in all cows after all services were given (P<.05). Probabilities of increasing pregnancy were enhanced in all groups if pregnancy was established after one service as compared to two services (P<. I 0). These data indicate that evaluation of HBP-B5 profiles in frozen bovine semen was related to fertility after Al in non-lactating cows. Pregnancy probabilities also increase as cow body condition increases in lactating and all cows, and also increase in cows that conceive after one service.
Description
Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.Includes bibliographical references: p. 45-51.
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Citation
Harris, Matthew Duncan (1996). Conception rates after artificial insemination as affected by heparin binding protein-B5 in frozen semen. Master's thesis, Texas A&M University. Available electronically from https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1996 -THESIS -H3663.
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