Molecular population genetics and epidemiology of Ceratocystis fagacearum
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The population genetic structure of Ceratocystis fagacearum was studied with genetic markers to address questions concerning the microgeographical spread and genetic variability of the oak wilt pathogen. Genetic diversity was assessed using restriction fragment length polymorphisms (RFLPs) and anonymous RFLP loci of the nuclear DNA (nuDNA) . Results of the study revealed only 4 haplotypes existing in the 3 populations analyzed. Of the 3 populations studied, 2 were asexually expanding and the other population represented a sexually active disease center. Three polymorphic RFLP loci were found. Only one population, Willow Springs, had more than 1 allele present at the RFLP loci. Very low levels of variation (G = 1, 1, and 11.69) were detected in the nuDNA among 96 isolates, from 3 distinct populations, of C. fagacearum. Hypotheses are discussed to account for this low level of genetic variation.
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Ivors, Kelly Lynn (1995). Molecular population genetics and epidemiology of Ceratocystis fagacearum. Master's thesis, Texas A&M University. Available electronically from