NOTE: This item is not available outside the Texas A&M University network. Texas A&M affiliated users who are off campus can access the item through NetID and password authentication or by using TAMU VPN. Non-affiliated individuals should request a copy through their local library's interlibrary loan service.
Screening, selection and evaluation of gamma irradiation-induced mutants of Zea mays L. for resistance to southern corn leaf blight
Abstract
The maternally inherited pollen-sterility character used prior to 1970 in commercial production of maize (Texas male-sterile system) conditions a hybrids' reaction to Bipolaris (Helminthosporium) maydis race T. Although certain hybrids and inbreds are more susceptible than others, any Texas male-sterile cytoplasm (cms-T) is more susceptible than its counterpart in the wild type, or normal, cytoplasm. Genes which restore pollen fertility, do not change this cytoplasmic susceptibility. The objective of this study was to induce durable and effective resistance to the disease caused by this organism, southern corn leaf blight, in an agronomically useful genetic background. Kernels of the cms-T version of an inbred with restored fertility, Tx441Rf, were exposed to four dosages of gamma-irradiation from ('60)Co. Dosages were subsequently evaluated for their efficiency and effectiveness in producing chlorophyll mutations. Both efficiency and effectiveness increased with increasing dosage based on the biological criteria used in calculations. First generation (M(,1)) plants were self-pollinated and their progeny evaluated for resistance to southern corn leaf blight. M(,2) and M(,3) seedlings were screened for resistance in a field trial (natural infection) in 1979 and in the greenhouse (controlled inoculation) in 1981. Intense selection pressure was applied in controlled inoculations with B. maydis race T by providing ideal conditions for disease development. Progeny of two resistant selections from the field trial exhibited resistance following the controlled inoculation. Four additional putative mutants with superior resistance were selected from among inoculated M(,2) seedlings. These resistant variants were evaluated in family studies where the frequency of chloroplast defects and viviparous mutants in the irradiated population was recorded. Success in the prescribed genetic manipulation of an agronomically important plant species can served as a prototype and speed progress in attaining disease resistance in maize and other crops. Our present understanding of this host-parasite interaction and mitochondrial membrane permeability is advanced by the discovery of phytotoxicity to s-triazine herbicides in cms-T maize. In vivo and in vitro studies demonstrated a greater sensitivity to atrazine and simazine for certain cms-T inbreds than for inbreds in normal cytoplasm.
Description
Includes bibliographical references (leaves 82-95)Subject
Plant Pathology1981 Disser-tation T917
Corn--Disease and pest resistance
Plant mutation breeding
Plants--Effect of gamma rays on
Plant Pathology
Collections
Citation
Tuleen, Darlene Mae (1981). Screening, selection and evaluation of gamma irradiation-induced mutants of Zea mays L. for resistance to southern corn leaf blight. Doctoral dissertation, Texas A&M University. Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -98875.
Request Open Access
This item and its contents are restricted. If this is your thesis or dissertation, you can make it open-access. This will allow all visitors to view the contents of the thesis.