Show simple item record

dc.contributor.advisorHalliwell, Robert S.
dc.creatorRussell, Thomas Edward
dc.date.accessioned2020-01-08T18:10:29Z
dc.date.available2020-01-08T18:10:29Z
dc.date.created1971
dc.date.issued1970
dc.identifier.urihttps://hdl.handle.net/1969.1/DISSERTATIONS-179607
dc.description.abstractLeaf tissue derived from a systemic host N. tabacum L. var. Samsun and a local lesion host N. tabacum I. var. Samsun NN of tobacco mosaic virus (TMV) was cultured in a modified Murashige and Skoog's liquid medium (M&S). Cell chains were isolated eight to fifteen days after culturing and subsequently sub-cultured in Leitz micro-culture dishes containing 25 μl of conditioned M&S medium surrounded by mineral oil. The cell chains had four to fourteen cells per chain. Two cell chains were placed in each micro-culture dish and one cell of one chain was micro-injected with either infectious or heat-treated TMV, and the other served as an uninjected control. Cells were injected with the aid of heat drawn glass needles, mounted in a micro-manipulator. Forty eight Samsun NN cell chains were injected. Twenty four injected cell chains were lost to injection damage and culture problems, nine chains became infected, two chains showed no symptoms and/or sign of infection, three cell chains were injected with heat-treated virus, and ten were used for other purposes. Twenty two cell chains of the Samsun variety were injected. All ten cell chains injected with infectious TMV became infected, five were injected with heat-treated TMV, and seven were lost or used for other purposes..en
dc.format.extent165 leaves : illustrationsen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectPlant Pathologyen
dc.subject.classification1970 Dissertation R967
dc.titleComparative cytopathology of cultured cells of a systemic host (Nicotiana tabacum L. var. Samsun) and a local lesion host (Nicotiana tabacum L. var. Samsun NN) micro-injected with tobacco mosaic virusen
dc.typeThesisen
thesis.degree.disciplinePlant Pathologyen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberApplegate, Howard G.
dc.contributor.committeeMemberFoster, William G.
dc.contributor.committeeMemberFrederiksen, Richard A.
dc.contributor.committeeMemberMorgan, Page W.
dc.type.genredissertationsen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen
dc.publisher.digitalTexas A&M University. Libraries


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

This item and its contents are restricted. If this is your thesis or dissertation, you can make it open-access. This will allow all visitors to view the contents of the thesis.

Request Open Access