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dc.contributor.advisorSimmons, Dick B.
dc.creatorGrimes, James Edward
dc.descriptionIncludes bibliographical references (leaves 59-63)en
dc.description"Submitted to the Graduate College of the Texas A & M University in partial fulfillment of the requirements for the degree of Doctor of Philosophy August 1967"en
dc.description.abstractThe purpose of this study was to prepare antigens from cell culture grown ornithosis agents. Special emphasis was placed on the development of an agglutination antigen for possible use in the detection of ornithosis infections in turkeys. McCoy cell monolayers were used for propagation of ornithosis agents which were maintained in the allantoic cavity of embryonated chicken eggs for seed stock. High yield of particles were obtained from cell cultures inoculated with the Texas turkey (TT) ornithosis agent or a white-winged dove isolate. The Jo strain did not produce usable quantities of particles. In the preparation of agglutination antigens, fluids from inoculated cell cultures were pooled, boiled, and treated with phenol. After cell culture debris was removed by centrifugation, particles were sedimented by centrifugation, re-suspended in distilled water, and re-sedimented. Washed particles were suspended in phosphate-buffered saline or borate-buffered saline (BBS) containing phenol. These antigens became inactive after prolonged storage. Some harvests of washed particles were suspended in BBS containing formaldehyde and, after storage, were re-sedimented then suspended in BBS containing phenol. The latter type of antigen reacted better in agglutination tests and results were more easily interpreted than were those using antigens prepared by the first method. ...en
dc.format.extent66 leaves : illustrationsen
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.subjectPsittacosis--prevention & controlen
dc.subject.classification1967 Dissertation G862
dc.subject.meshPoultry Diseases--microbiologyen
dc.titleAntigen preparation from ornithosis agents grown in cell culture : a dissertationen
dc.typeThesisen Microbiology.en Microbiologyen A&M Universityen of Philosophyen
dc.contributor.committeeMemberAberth, Oliver
dc.contributor.committeeMemberAnderson, Robert
dc.contributor.committeeMemberColunga, Daniel
dc.contributor.committeeMemberRhyne, V. Thomas
dc.format.digitalOriginreformatted digitalen
dc.publisher.digitalTexas A&M University. Libraries

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