Abstract
The purpose of this study was to prepare antigens from cell culture grown ornithosis agents. Special emphasis was placed on the development of an agglutination antigen for possible use in the detection of ornithosis infections in turkeys. McCoy cell monolayers were used for propagation of ornithosis agents which were maintained in the allantoic cavity of embryonated chicken eggs for seed stock. High yield of particles were obtained from cell cultures inoculated with the Texas turkey (TT) ornithosis agent or a white-winged dove isolate. The Jo strain did not produce usable quantities of particles. In the preparation of agglutination antigens, fluids from inoculated cell cultures were pooled, boiled, and treated with phenol. After cell culture debris was removed by centrifugation, particles were sedimented by centrifugation, re-suspended in distilled water, and re-sedimented. Washed particles were suspended in phosphate-buffered saline or borate-buffered saline (BBS) containing phenol. These antigens became inactive after prolonged storage. Some harvests of washed particles were suspended in BBS containing formaldehyde and, after storage, were re-sedimented then suspended in BBS containing phenol. The latter type of antigen reacted better in agglutination tests and results were more easily interpreted than were those using antigens prepared by the first method. ...
Grimes, James Edward (1967). Antigen preparation from ornithosis agents grown in cell culture : a dissertation. Doctoral dissertation, Texas A&M University. Texas A&M University. Libraries. Available electronically from
https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -170007.