Abstract
Gross and histologic lesions induced by different strains of Moloney murine sarcoma viruses (MoMuSVs) were compared. Neoplasms induced by MoMuSV-HT1 histologically resembled fibrosarcomas with occasional cells containing large nuclei. Neoplasms induced by MoMuSV-m1 were composed predominantly of spindle-shaped cells with small vascular spaces. Mice infected with MoMuSV-HT1 and MoMuSV-m1 had dense splenic infiltrates of primitive blast cells effacing the normal architecture. Neoplasms induced by MoMuSV-124 and MoMuSV-349 were indistinguishable and best described as angioproliferative. Temporal studies revealed that mice inoculated with MoMuSV-349 developed neoplasms between 7 and 14 days post-inoculation (PI). Neoplasms detected at the earlier time points (14-21 days PI) were composed exclusively of spindle-shaped cells. Neoplasms at later time points contained large vascular spaces lined by a hyperplastic endothelium. These results strongly suggested neoplasms induced by MoMuSV-349 had a changing histologic appearance. As the disease induced by MoMuSV-349 progressed, mice developed significant thymic atrophy, neutrophilia, lymphopenia and anemia. Non-radioactive in situ hybridization revealed specific target cells infected with MoMuSV-349. The tumor cells that demonstrated positive hybridization were initially the spindle-shaped cells and finally the endothelial cells. The results obtained by in situ hybridization were confirmed by the polymerase chain reaction assay. Cell lines were established from neoplasms to determine if neoplastic cells secreted fibroblast growth factor(s). Conditioned media from the tumor cell lines stimulated mitogenesis in mouse fibroblasts and proliferation of bovine endothelial cells. This ability to stimulate proliferation of endothelial cells was partially blocked by incubating the conditioned medium with a neutralizing basic FGF (bFGF) antibody and was eliminated by binding the medium to heparin Sepharose (HS) beads...
Hoffman, Robert Jay (1992). A morphologic, hematologic and molecular study of Moloney murine sarcoma virus 349-induced tumors in BALB/c mice. Texas A&M University. Texas A&M University. Libraries. Available electronically from
https : / /hdl .handle .net /1969 .1 /DISSERTATIONS -1348955.