Activation of the Cell-Mediated Response in Guinea Pigs Via Subcellular Units of Listeria Monocytogenes
Abstract
The purpose of this study was to investigate the cell-mediated activity of the facultative intracellular parasite, Listeria Monocytogenes, in the guinea pig. Special emphasis was placed upon the particular subcellular component responsible for the demonstration of the two lymphokines: Migration Inhibitory Factor and Skin Reactive Factor.
In order to isolate the specific subcellular components it was necessary to fractionate the live organism. This was accomplished using a variety of methods. When these antigenic preparations were injected into guinea pigs immunized with live Listeria Monocytogenes, definite skin test reactions were noted. These same preparation, when employed in the capillary tube technique for assaying macrophage migration, demonstrated a marked inhibition of macrophage migration. These crude preparations evidently contain the specific subcellular component responsible for the demonstration of the cell-mediated response in guinea pigs.
Description
Program year: 1976-1977Digitized from print original stored in HDR
Citation
Sheffy, Deborah A. (1977). Activation of the Cell-Mediated Response in Guinea Pigs Via Subcellular Units of Listeria Monocytogenes. University Undergraduate Fellows. Available electronically from https : / /hdl .handle .net /1969 .1 /CAPSTONE -SheffyD _1977.