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dc.contributor.advisorEbbole, Daniel J.
dc.creatorLi, Dan
dc.date.accessioned2006-08-16T19:10:08Z
dc.date.available2006-08-16T19:10:08Z
dc.date.created2003-05
dc.date.issued2006-08-16
dc.identifier.urihttp://hdl.handle.net/1969.1/3936
dc.description.abstractMAP kinases and transcription factors homologous to Saccharomyces cerevisiae Fus3p/Kss1p and Ste12p have been identified in several plant pathogenic fungi and found to be required for pathogenicity and sexual reproduction. A gene encoding the homolog of Fus3p/Kss1p in Neurospora crassa was isolated previously and named mak- 2 (mitogen activated kinase -2). This dissertation describes the isolation of the Ste12p homolog, pp-1 (protoperithecia-1) and the comparison of the phenotypes of the mak-2 and pp-1 mutants. The similar phenotypes of the mak-2 and pp-1 null mutants suggest that these proteins are part of the same MAP kinase signaling cascade in N. crassa. In addition to reduced growth rate, the phenotypes of the mutants demonstrate that this pathway is required for female fertility, contributes to aerial hyphal development and repression of conidiophore development. The mak-2 MAP kinase pathway also regulates several genes putatively involved in secondary metabolism during the mating process. Among these is a gene cluster that is likely to be involved in the production of a polyketide secondary metabolite. An orthologous gene cluster was also identified in M. grisea, and the structural and functional homology of these two related gene clusters was characterized. Microarray analysis was used to extend the analysis of gene expression in mak-2 and pp-1 mutants, and a number of downstream target genes of the MAP kinase pathway were identified and called mak-2 kinase-regulated genes (mkr). A model of this MAP kinase pathway in N. crassa was developed. Since N. crassa is a saprophytic fungus but closely related to several plant pathogens, this research may provide an important perspective on the evolution of a major regulatory pathway governing fungal pathogenesis.en
dc.format.extent17443027 bytesen
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.subjectMAP KINASE PATHWAYen
dc.subjectNEUROSPORA CRASSAen
dc.titleA map kinase pathway essential for mating and contributing to asexual development in Neurospora Crassaen
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentPlant Pathology and Microbiologyen
thesis.degree.disciplinePlant Pathologyen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameDoctor of Philosophyen
thesis.degree.levelDoctoralen
dc.contributor.committeeMemberGolden, James W.
dc.contributor.committeeMemberScholthof, Herman B.
dc.contributor.committeeMemberWilkinson, Heather H.
dc.type.genreElectronic Dissertationen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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