Annexation of Interchromosomal Space During Viral Infection of 3D Tissue Matrix
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The process of viral infection has been shown to be a tightly structured process that exerts precise control over the nucleus. By quantitatively documenting the dynamic nuclear changes in response to nuclear infection of cells suspended in a three-dimensional collagen matrix, an understanding of nuclear reorganization can be elucidated from the tissue. Tissues were cultured in vitro in three dimensional collagen matrices which act as scaffolding that mimics the native environment. The cells were then infected with cytomegalo virus (CMV) which was labeled with green fluorescent protein to allow identification of the virus. During viral infection, nonlinear optical microscopy (NLOM) was employed to nondestructively image the nuclear volume over time. Comparing changes in the nuclear volume with control values, we expected to identify several stages of nuclear reorganization that have previously been identified during infection of 2D tissue cultures. Once it has been demonstrated that NLOM can be successfully used to track dynamics of infection, multiple mutant fluorescent proteins can be used to track host, virus and immune response to viral infection.
Crannell, Zachary (2006). Annexation of Interchromosomal Space During Viral Infection of 3D Tissue Matrix. Available electronically from