Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors
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Provided are methods and compositions for increasing the efficiency of nuclear transfer using apoptosis inhibitors, and for the production of transgenic and non-transgenic mammals from cultured cells or cell lines. Methods for cloning mammals, and for producing transgenic and chimeric mammalian tissues and mammals, and chimeric cell lines are also provided.
Piedrahita, Jorge A.; Lee, Chang-kyu; Weaks, Regina; Bazer, Fuller (2003). Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from