Cells and methods for the generation of transgenic pigs
Abstract
Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.
Subject
800/21435/325
435/383
435/384
435/455
435/459
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435/462
435/463
800/14
800/15
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A01K 67/0275
C12N 5/0611
A01K 2217/05
A01K 2217/075
C12N 2500/24
C12N 2500/32
C12N 2501/115
C12N 2501/125
C12N 2501/235
C12N 2510/00
C12N 2517/04
C12N 2517/10
C12N 2502/13
Collections
Citation
Piedrahita, Jorge A.; Bazer, Fuller W. (2001). Cells and methods for the generation of transgenic pigs. United States. Patent and Trademark Office; Texas A&M University. Libraries. Available electronically from https : / /hdl .handle .net /1969 .1 /176670.