Prompt and In Situ Diagnosis of Live/Dead Bacteria
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The goal of this project is to develop a method for rapid and in situ identification of live and dead bacteria using absorption spectroscopy, emission spectroscopy, and principal component analysis. To achieve this goal, we have designed a portable, handheld fluorescence spectrometer utilizing a UV-diode laser as an excitation source and a CCD for detection. This work presents evidence that principle component analysis can be used to successfully differentiate living from dead bacteria on site, quickly, with hand-held, portable equipment.
Walck, Matthew Alexander (2017). Prompt and In Situ Diagnosis of Live/Dead Bacteria. Undergraduate Research Scholars Program. Available electronically from