Genetic Dissecting Innate Immune Signaling Networks in Arabidopsis
MetadataShow full item record
The plant innate immune system is built on a great number of processes, many of which are uncharacterized, or incompletely identified. Infection of Arabidopsis thaliana with bac- terium Pseudomonas syringae pv. tomato DC3000 carrying effector proteins AvrRpm1 and AvrRpt2, is known to elicit effector-triggered immune response (ETI) via interaction with host-cell receptor proteins RPM1 and RPS2, respectively. Recognition of pathogen- associated molecular patterns (PAMPs), and pathogen effector proteins initiate a variety of biochemical signaling events ultimately resulting in physiological changes favoring the host’s self-preservation. To better characterize those signaling pathways, we examined in- duced expression levels of immune gene transcription factor (TF) WRKY46 in mutagenized transgenic pWRKY46-LUC (WRKY46 TF fused with luciferase reporter) A. thaliana model organisms. Here, we show that the WRKY46 TF is induced by inoculation and putative subsequent elicitation of PTI and/or ETI within mutagenized plants by unknown mech- anisms. Genetic dissection of the pathways leading to such responses will permit future characterization of the underlying mechanisms and sequencing of the associated host genes.
Provasek, Vincent (2014). Genetic Dissecting Innate Immune Signaling Networks in Arabidopsis. Undergraduate Research Scholars Program. Available electronically from