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dc.contributor.advisorMaitland, Kristen C
dc.creatorBixler, Joel Nathan
dc.date.accessioned2015-04-28T15:21:25Z
dc.date.available2016-12-01T06:36:11Z
dc.date.created2014-12
dc.date.issued2014-08-27
dc.date.submittedDecember 2014
dc.identifier.urihttp://hdl.handle.net/1969.1/153844
dc.description.abstractWe have integrated a fluorescence microendoscope into a whole-animal optical imaging system, allowing for simultaneous microscopic and macroscopic imaging of tdTomato expressing BCG in vivo. A 535 nm LED was collimated and launched into a 10,000 element fiber bundle can be inserted through an intra-tracheal catheter into the lung of a mouse. Fluorescence emission can either be (1) collected by the bundle and imaged onto the surface of a CCD camera for localized detection or (2) the fluorescence can be imaged by the whole animal imaging stystem providing macroscopic information. Results from internal localized excitation and external whole body detection indicate the potential for imaging bacterial infections down to 100 colony forming units. This novel imaging technique has the potential to allow for functional studies, enhancing the ability to assess new therapeutic agents.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectFluorescence spectroscopyen
dc.subjectBacterial Imagingen
dc.subjectTuberculosisen
dc.subjectmicroendoscopeen
dc.titleMulti-Scale Imaging of Respiratory Bacterial Infection Using Fiber Microendoscopy and Whole-Animal Imagingen
dc.typeThesisen
thesis.degree.departmentBiomedical Engineeringen
thesis.degree.disciplineBiomedical Engineeringen
thesis.degree.grantorTexas A & M Universityen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
dc.contributor.committeeMemberYakovlev, Vladislav V
dc.contributor.committeeMemberCirillo, Jeffrey D
dc.type.materialtexten
dc.date.updated2015-04-28T15:21:26Z
local.embargo.terms2016-12-01
local.etdauthor.orcid0000-0002-9970-4141


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