Insights Into the Effect of Trigonelline in the P53 Pathway
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Colon cancer is the second leading cause of cancer-related deaths in the United States. Early screening has improved detection of precancerous polyps, however, colon cancer still claims over fifty thousand lives per year. Statistics have shown a strong correlation between an increased level of estrogen and a decreased risk of colon cancer. Data collected in our laboratory has shown that estradiol (E2) is protective through its ability to increase apoptosis of non-malignant colonocytes that are at risk for becoming cancerous due to stress. This is mediated through a mechanism involving estrogen receptor β (ER β) in mice, and the protein p53 has been found to be protective in some mechanisms. Trigonelline (Trig) is a product of niacin metabolism and accounts for ~1% dry matter in roasted coffee beans. Data from our laboratory has shown the ability of Trig to induce an estrogenic response through the activation of nuclear reporter assays. We investigated the effect of Trig by analyzing the apoptotic and growth activity of young adult mouse colonocytes (YAMCs) in response to non-permissive conditions and the role that the p53 protein plays in the apoptotic mechanism. RT-PCR analysis was used to measure protein levels that are associated with pro-apoptotic p53 target genes (PUMA, Bax, and Noxa) in YAMCs after treatment to demonstrate the protective estrogenic effect of Trig.
Justice, Jessica (2014). Insights Into the Effect of Trigonelline in the P53 Pathway. Honors and Undergraduate Research. Available electronically from