Co-Culture of Equine Mesenchymal Stem Cells with Synoviocytes to Modulate Induced Inflammation In-Vitro
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Stem cells are the current topic of interest when it comes to the forefront of tissue engineering and new nonivassive therapies; and at the pennacle of regenerative medicine for orthopedic tissues and immunomodulary treatments stands Mesenchymal Stem Cells (MSC). MSCs have demonstrated multiple therapeutic uses due to their abilities to both induce tissue regeneration in mutiple mesoderm derived cell lineages, as well as respond to and mediate cellular activity in their local environment. In order to better understand the ways in which MSCs act to mediate acute inflammation, an environmental scenario similar to that of osteoarthritis was created by inducing synovitis in-vitro. Equine synoviocytes were harvested, plated, and then exposed to the inflammatory cytokines TNF-Alpha and IL-1Beta. The synoviocytes suspended in the pro-inflammatory cytokines were then treated with autogenic MSCs in order to observe the mechanisms by which they would interact with the synoviocytes in order to regulate inflammation. The media containing both the mesenchymal stem cells and the inflamed synoviocytes was harvested and the concentration of extracellular PGE-2 was analyzed using ELISA in an effort to observe any communication between the two cell types. Synoviocyte proliferation was also observed visually by comparing colony formation and cell confluence prior to inflammation, following inflammation, and following MSC treatment. Further investigation must be done before definitive results can be described. Significant conclusions cannot be drawn based on the preliminary data that is currently available.
Fussell, Vincent (2014). Co-Culture of Equine Mesenchymal Stem Cells with Synoviocytes to Modulate Induced Inflammation In-Vitro. Honors and Undergraduate Research. Available electronically from