Now showing items 1-5 of 5

    • Characterization of Cas9-Mediated microRNA Knockout 

      Morin, Andrew (2015-11-18)
      MicroRNAs are short regulatory RNAs that primarily operate at the posttranscriptional level, acting as part of the RISC complex to destabilize mRNA prior to, or during, translation. MicroRNAs are subject to several levels ...
    • Efficiency Prediction and Mechanism Discovery for the CRISPR-Cas9 System 

      Yan, Yi (2018-05-08)
      CRISPR-Cas9 has been employed as a genome editing tool in a wide range of cells of different organisms. One of the biggest challenges it faces is to maintain the efficiency of the gene regulation. To address this challenge, ...
    • The Roles of Sox2 and Sox3 During Otic and Epibranchial Development in Zebrafish 

      Gou, Yunzi (2019-04-29)
      Sox2 and Sox3 are SoxB1 transcription factors that act redundantly in the neural ectoderm, but also are uniquely required in other systems. To address functions of sox2 and sox3 in otic and epibranchial development, we ...
    • Tobacco Mosaic Virus as a Gene Editing Platform 

      Chiong, Kelvin Tak Lek (2018-06-28)
      Plant viruses can be engineered and utilized as transient tools for foreign gene overexpression studies and large-scale production of high value proteins. Tobacco mosaic virus (TMV) has been widely used for these purposes. ...
    • A Viral-Based Toolbox for Efficient Gene Editing in Nicotiana Species 

      Cody, Will Bryan (2018-07-27)
      The CRISPR/Cas9 site-specific nuclease from a prokaryotic viral defense mechanism has been adapted into a gene editing tool for molecular biologists. Consisting of two deliverable parts, a Cas9 nuclease and sequence specific ...