Browsing by Author "Siegele, Deborah"
Now showing items 1-20 of 20
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Adaptive Evolution for the Study of Complex Phenotypes in Microbial Systems
Reyes Barrios, Luis Humberto (2013-07-24)Microbial-based industrial production has experienced a revolutionary development in the last decades as chemical industry has shifted its focus towards more sustain- able production of fuels, building blocks for materials, ... -
Bacteriophage Lambda Integrates Microenvironments, Individuality, and Interactions to Formulate Subcellular Decisions
Trinh, Jimmy Tri (2018-05-04)Decision-making influences the development of life over multiple levels. Understanding the core mechanisms of this complex process could yield new insights into the treatment of diseases and the evolution of lifeforms. To ... -
Bacteriophage ms2 l protein: genetic and biochemical characterization
McIntosh, Brenley Kathleen (2009-05-15)In order to release progeny, bacteriophages must lyse the host cell by compromising the peptidoglycan layer. There are two known strategies of lysis: the holin-endolysin system and single gene lysis (SGL), which are dependent ... -
Bacteriophage P1: a new paradigm for control of phage lysis
Xu, Min (Texas A&M University, 2005-11-01)The N-terminal hydrophobic domain of the phage P1 endolysin Lyz was found to facilitate the export of Lyz in a sec-dependent fashion, explaining the ability of Lyz to cause lysis of E.coli in the absence of the P1 holin. ... -
Bacteriophage T4 lysis and lysis inhibition: molecular basis of an ancient story
Tran, Tram Anh Thi (2009-05-15)T4 requires two proteins: holin, T (lesion formation and lysis timing) and endolysin, E (cell wall degradation) to lyse the host at the end of its life cycle. E is a cytoplasmic protein that sequestered away from its ... -
Charactering Recombinants with Multiple Complex Phenotypes Developed Using the Genderless Recombination System
Li, Tianyu (2020-04-16)Strain robustness and productivity are key traits for bio-based production. Stressors, such as inhibitory compounds present in the feedstock, osmotic, temperature, and toxic products, can inhibit cell growth and productivity. ... -
Complex Organization and Dynamic Regulation of the pks Gene Cluster in Bacillus subtilis
Vargas Bautista, Carol M (2014-08-27)The pks genes are the largest antibiotic- encoding gene cluster in Bacillus subtilis and encode the Pks enzymatic complex that produces bacillaene. Bacillaene plays important roles in the fitness of B. subtilis during ... -
Development of rapid microbial methods for lysine quantification in feed ingredients based on green fluorescent protein fluorescence
Chalova-Zhekova, Vesela I. (Texas A&M University, 2007-04-25)Lysine is one of the more limiting amino acids in protein sources for chickens. Since lysine is also an essential amino acid for animals, it is an important component of animal dietary formulation. Therefore, an accurate ... -
Functional proteomics in Escherichia coli
Champion, Matthew Maurice (Texas A&M University, 2006-04-12)Cells respond to their environment with programmed changes in gene expression. Cataloging these changes at the protein level is key towards understanding the physiology of an organism. Multi-subunit and multi-protein ... -
Identification of novel regulatory mechanisms controlling heterocyst development in Anabaena Sp. strain PCC 7120
Aldea, Maria Ramona (2009-05-15)The regulatory mechanisms that govern heterocyst development in Anabaena sp. strain PCC 7120 have been continuously refined over the last two decades. In this work, we show that three of the sigma factor genes present in ... -
Kinetics and dynamics study on the allosteric pathway of phosphofructokinase from Escherichia coli
Tie, Cuijuan (Texas A&M University, 2008-10-10)Phosphofructokinase from Escherichia coli (EcPFK) is allosterically regulated by MgADP and phosphoenolpyruvate (PEP), which act to activate or inhibit, respectively, by changing the substrate (Fru-6-P) affinity of the ... -
An ontology for microbial phenotypes
Chibucos, Marcus; Zweifel, Adrienne; Herrera, Jonathan; Meza, William; Eslamfam, Shabnam; Uetz, Peter; Siegele, Deborah; Hu, James; Giglio, Michelle (BMC Microbiology, 2014) -
Organization of the circadian clock and control of rhythmicity in fungi
Greene, Andrew Vanderford (Texas A&M University, 2006-10-30)Circadian rhythms in biological processes occur in a wide range of organisms and are generated by endogenous oscillators. In Neurospora crassa, the FRQ-oscillator (comprised of FRQ, WC-1 and WC-2) is essential for rhythms ... -
Regulation of Chromosome Replication and Segregation during Bacillus subtilis Sporulation
Duan, Yi (2016-12-08)Bacteria precisely coordinate DNA replication and chromosome segregation with cell division; accurate regulation of each of these processes is critical to genome inheritance and thus reproductive success. During fast growth, ... -
Regulation of Phage Lysis: Diverse Strategies and Targets
Chen, Yi (2016-08-01)Most of the large, double-stranded DNA (dsDNA) bacteriophages infecting Gram-negative hosts utilize multi-protein strategy to lyse the three layers of the host cell envelop: the inner membrane (IM), the peptidoglycan (PG), ... -
Regulation of the Nitrogen Fixation Genes in the Heterocystous Cyanobacterium Anabaena sp. Strain PCC 7120
Kumar, Krithika (2012-02-14)Many multicellular cyanobacteria produce specialized nitrogenfixing heterocysts. During diazotrophic growth of Anabaena (Nostoc) sp. strain PCC 7120, a regulated developmental pattern of single heterocysts separated by ... -
Significance of Amino Acid(S) During Clostridium Difficile Spore Germination
Shrestha, Ritu (2019-01-16)Clostridium difficile spore germination is critical for the transmission of disease. C. difficile spores germinate in response to cholic acid derivatives, such as taurocholate (TA), and amino acids, such as glycine or ... -
Single-Cell and Single-Molecule Studies of Bacteriophage Lambda Post-Infection Decision-Making
Shao, Qiuyan (2017-12-01)Cellular decision making is a ubiquitous process among all life forms, and a key step that organisms take to integrate the environmental signals to choose an optimal response to improve their overall fitness. The genetic ... -
Tryptophan Regulation of the Escherichia coli Tryptophanase (TNA) Operon
Martinez, Allyson Kendall (2013-10-22)Free L-tryptophan induces the expression of the Escherichia coli tna operon that specifies proteins necessary for catabolizing tryptophan. Regulation is effected by a transcriptional attenuation mechanism requiring ... -
What makes the lysis clock tick? A study of the bacteriophage holin
White, Rebecca Lynn (2009-05-15)The timing of host lysis is the only decision made in the bacteriophage lytic cycle. To optimize timing, double-stranded DNA phages use a 2-component lysis system consisting of a muralytic enzyme, the endolysin, and a small ...