Browsing by Author "Smith, Gale E."

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    A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.
    (Texas Agricultural Experiment Station, 1987) Summers, Max D.; Smith, Gale E.
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    Method for producing a recombinant baculovirus expression vector
    (United States. Patent and Trademark Office, 1988-05-17) Smith, Gale E.; Summers, Max D.; The Texas A&M University System
    A method for producing a recombinant baculovirus expression vector, capable of expression of a selected gene in a host insect cell, is disclosed. The preferred method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant shuttle vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant shuttle vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome.
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    Method for producing a recombinant baculovirus expression vector
    (United States. Patent and Trademark Office, 1989-11-07) Smith, Gale E.; Summers, Max D.; The Texas A&M University System
    A method for producng a recombinant baculovirus expression vector, capable of expressing a selected gene in a host insect cell, is disclosed. The method involves cleaving baculovirus DNA to produce a DNA fragment comprising a polyhedrin gene or portion thereof, including a polyhedrin promoter. A recombinant transfer vector is prepared by inserting said DNA fragment into a cloning vehicle and thereafter inserting a selected gene into the thus modified cloning vehicle such that it is under the transcriptional control of the polyhedrin promoter. The recombinant transfer vector is contacted with a baculovirus DNA so as to effect recombination and incorporation of the selected gene into the baculovirus genome. The resultant recombinant baculovirus is then used to infect susceptible insects or cultured insect cells and the protein product from the incorporated selected gene is produced from the infection.