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dc.contributor.advisorVersaw, Wayne
dc.creatorGuo, Biwei
dc.date.accessioned2010-01-15T00:07:05Z
dc.date.accessioned2010-01-16T00:48:25Z
dc.date.available2010-01-15T00:07:05Z
dc.date.available2010-01-16T00:48:25Z
dc.date.created2008-05
dc.date.issued2009-05-15
dc.identifier.urihttps://hdl.handle.net/1969.1/ETD-TAMU-2662
dc.description.abstractThe transport of phosphate (Pi) between subcellular compartments is central to metabolic regulation. Although some of the transporters involved in controlling the intracellular distribution of Pi have been identified in plants, others are predicted from genetic and biochemical studies. The Arabidopsis thaliana genome encodes a family of six proteins that share similarity with SLC17/type I Pi transporters, a diverse group of animal proteins involved in the transport of Pi, organic anions and chloride. Heterologous expression in yeast, and gene expression and localization studies in plants were used to characterize all six members of this Arabidopsis family, which we have named PHT4. All of the PHT4 proteins mediate Pi transport in yeast with high specificity. Bioinformatic analysis and localization of PHT4-GFP fusion proteins indicate that five of the proteins are targeted to the plastid inner envelope membrane, and the sixth resides in the Golgi apparatus. PHT4 genes are expressed in both roots and leaves although two of the genes are expressed predominantly in leaves and one mostly in roots. These expression patterns, together with Pi transport activities and subcellular locations, suggest roles for PHT4 proteins in the transport of Pi between the cytosol and chloroplasts, heterotrophic plastids and the Golgi apparatus.en
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.subjectArabidopsisen
dc.subjectchloroplasten
dc.subjectGolgien
dc.subjectheterotrophic plastiden
dc.subjectphosphate transporteren
dc.titleFunctional analysis of the Arabidopsis PHT4 family of intracellular phosphate transportersen
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiologyen
thesis.degree.disciplineBiologyen
thesis.degree.grantorTexas A&M Universityen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
dc.contributor.committeeMemberHirschi, Kendal
dc.contributor.committeeMemberMcKnight, Thomas
dc.contributor.committeeMemberRiley, Bruce
dc.type.genreElectronic Thesisen
dc.type.materialtexten
dc.format.digitalOriginborn digitalen


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