LUMINESCENCE LIFETIME INSTRUMENTATION DEVELOPMENT FOR MULTI-DYE ANALYSIS
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An efficient and accurate luminescence based instrument capable of determining differences in lifetime decay emissions of multiple dyes by using a new multiluminophore lifetime calculation method was developed for eventual use in dual sensing applications. Current methods for monitoring multiple luminescent dyes, such as dual lifetime determination (DLD), do not accurately calculate actual lifetimes. In this work, a mathematical model of the system was produced by using two different computer programs in order to simulate variables and to develop an efficient and accurate method for simultaneously calculating the lifetimes. The calculation method was based on a new correction algorithm recently developed in the research group. Using these models and optical hardware, an instrument was created to be driven by a personal computer equipped with custom LabVIEW software, which also analyzed the recorded data. During testing, the system was able to accurately calculate the lifetimes of two distinct luminophores. It was determined that this system is advantageous over current multi-dye analysis techniques by providing accurate and computationally-efficient calculations with the potential of implementing low-cost materials in the future. This system could eventually be implemented for many dual-sensing applications, where two parameters must be monitored at once. For example, patients suffering from diabetes could use a non-invasive monitor based on this system to detect varying tissue oxygen levels to compensate for enzymatic glucose sensor response.
Shadfan, Adam (2011). LUMINESCENCE LIFETIME INSTRUMENTATION DEVELOPMENT FOR MULTI-DYE ANALYSIS. Texas A&M University. Available electronically from