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dc.creatorWang, Jason
dc.date.accessioned2013-02-22T20:39:50Z
dc.date.available2013-02-22T20:39:50Z
dc.date.created2004
dc.date.issued2013-02-22
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-2004-Fellows-Thesis-W26
dc.descriptionDue to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.en
dc.descriptionIncludes bibliographical references (leaves 26-27).en
dc.description.abstractNuclear pore complexes (NPCs) facilitate the bidirectional transport of a myriad of molecules, ranging in size from an ion to assembled ribosomal subunits, across the two lipid bilayers that constitute the nuclear envelope (NE). Research over the past decade has imparted much insight into how NPCs control the selective passage of large macromolecules while maintaining their compartmentalization properties. Schemes for soluble cofactor-mediated active transport have been developed through phenotypic mutational analyses and in vitro experiments. Continuing the effort for a more comprehensive characterization of NPC function, we have developed a method for the in vivo analysis of nucleocytoplasmic transport utilizing fluorescently labeled, recombinant human ribosomal protein L23a (rpL23a-His). Cytoplasmic microinjections into live HeLa cells indicated rapid nuclear import and subsequent export of rpL23a-His. These results are expected to lead to real-time in vivo single-molecule observation of ribosomal subunit trafficking and assembly.en
dc.format.mediumelectronicen
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.publisherTexas A&M University
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en
dc.subjecten
dc.subjectMajors genetics and biochemistry.en
dc.titleReal-time trafficking and assembly of ribosomal subunitsen
thesis.degree.departmenten
thesis.degree.disciplineen
thesis.degree.nameFellows Thesisen
thesis.degree.levelUndergraduateen
dc.type.genrethesisen
dc.type.materialtexten
dc.format.digitalOriginreformatted digitalen


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