The effects of the trol4 and trols1 mutations on neural stem cell development and proliferation in Drosophila melanogaster embryos

Abstract

The locus terribly reduced optic lobes or trol, is an X-linked homeobox gene involved in the control of neuroblast proliferation in Drosophila melanogaster larvae. It is known that trol acts in a pathway to counteract cell cycle repressors via the induction of cyclin E expression. The goal of the project is to use immunohistochemical methods to attempt to establish a phenotype for trol in the embryonic stage of development. Using the anti-engrailed and anti-fasciclin III antibodies, the developing nervous system can be examined for physical differences between different alleles and the wildtype embryos.