Growth and plasmid characteristics of a porcine-derived Salmonella typhimurium resistant to chlortetracycline
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This study was a preliminary investigation in which porcine derived Salmonella typhimurium was selected for resistance to the equivalent of 20o and 400 grams of chlortetracydine per ton of swine feed. These resistant strains were cultured at 25₉C and 37₉C, serially diluted, plated, counted, and compared to the non-resistant control. Strain viable cell counts were lower in the resistant strains than in the control. However, viable cell counts in the resistant groups was about the same at both temperatures as were the viable cell counts of the control. Another preliminary study that intended to show the abilities of the resistant strains to transfer resistance to the non-resistant strain gave unexpected results. Plasmids were isolated for the strains (i.e., control, 200g/ton CT-resistant, and 400g/ton CT-resistant) and the two resistant strains that had been cultured in continuous-flow cultures of porcine fecal bacteria. The Plasmids were electrophoresed on agarose gels. While all five strains had plasmids, one of the strains cultured in the continuous-flow culture had additional plasmids when not detected in the preparations.
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Includes bibliographical references: leaves 11-12.
Shiflett, J. Mason (1998). Growth and plasmid characteristics of a porcine-derived Salmonella typhimurium resistant to chlortetracycline. Texas A&M University. Available electronically from