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dc.creatorSiddiqui, Najeeb Ullahen_US
dc.date.accessioned2012-06-07T22:50:30Z
dc.date.available2012-06-07T22:50:30Z
dc.date.created1997en_US
dc.date.issued1997
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-1997-THESIS-S564en_US
dc.descriptionDue to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionIssued also on microfiche from Lange Micrographics.en_US
dc.description.abstractWe studied the expression of three promoter deletion mutants (A218, A599, and A1312) of the Late Embryo Abundant (LEA) class gene Dc3 fused to the reporter gene, B-glucuronidase (GUS) in 14 d old transgenic tobacco seedlings. Seedlings were subjected to one of the following stresses: dehydration, hypoxia, salinity, exogenous ethylene, and exogenous methyl jasmonate (MeJa). GUS activity was quantified fluorometrically, and expression was observed by histochemical staining of the seedlings. An increase in GUS activity was observed in A599 and A1312 mutants in response to dehydration and salinity within 6 h of stress, and at 12 h in response to hypoxia. No increase in endogenous ABA was found in any of the three mutants even after 24 h of hypoxia. ABA-independent increase in GUS activity was observed when endogenous ABA biosynthesis was blocked by fluridone and plants were exposed to 5 [[ ]-' ethylene in air or 100 @M MeJa. No expression was observed in A218 mutant in response to dehydration, salinity, or MeJa. A mild response was observed in response to ethylene or hypoxia. This suggests that the region between-218 and-599 is absolutely necessary for ABA (dehydration and salinity) and MeJa-dependent expression whereas ethylene mediated expression does not require this region of the promoter. This study shows that Dc3, which is an ABA responsive gene, can also be induced by stress signals other than ABA.en_US
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en_US
dc.subjectplant physiology.en_US
dc.subjectMajor plant physiology.en_US
dc.titleAbscisic acid-dependent and -independent expression of the carrot LEA class gene Dc3 in transgenic tobacco seedlingsen_US
dc.typeThesisen_US
thesis.degree.disciplineplant physiologyen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelMastersen_US
dc.type.genrethesis
dc.type.materialtexten_US
dc.format.digitalOriginreformatted digitalen_US


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