Abstract
Serum total cholesterol (TC), lipoprotein cholesterol distribution, and lecithin:cholesterol acyltransferase (LCAT) activities of chronic renal failure dogs were investigated. Control dogs (n = 14) and affected dogs (n = 29) were fed one of three protein/phosphorous restricted diets: low protein canned (LPC), medium protein canned (MPC), or low protein dry (LPD), for 3 to 5 weeks. Basal diets were then supplemented for 6 weeks with menhaden fish oil (MHO) or safflower oil (SFO) in a crossover design which included a 3 week wash-out period. After basal diet acclimation, serum TC increased (paired t-test, p < 0.05) in LPC, MPC, and LPD control groups. At the same time, (X2 lipoprotein cholesterol increased (p < 0.05) in all three control groups. The MPC control dogs also showed an increase (p = 0.01) in cholesterol associated with the (xl fraction after diet acclimation. Among affected dogs, only those receiving the MPC diet showed an elevation (p < 0.01) in serum TC after the basal period. Significant MHO supplement effects included a decrease (p < 0.01) in serum TC in MPC control dogs, and a decrease (p < 0.05) in a2 lipoprotein cholesterol in LPD control dogs. Safflower oil had no significant effect on plasma lipoproteins. No significant differences in serum TC or lipoprotein cholesterol distribution were found between affected and control dogs regardless of basal diet or oil supplementation. Serum LCAT activates were assessed in a subset of MPC dogs; no significant differences between affected and control dogs were revealed. When these two groups were combined, ANOVA revealed an increase in LCAT activities (p < 0.03) after the basal period, and a significant decrease in LCAT activities (p < 0.05) after SFO supplementation. Previously, it was shown that the MPC diet elevated LCAT activities in normal dogs due to its higher fat content; the present group of control and affected dogs confirmed these findings. In the present study, LCAT activates of affected dogs may have been elevated prior to entry because these dogs had been fed renal failure diets which were relatively high in fat before entering the clinical trial.
Malcik, Kimberly L (1996). Lipoprotein metabolism and LCAT activity in chronic renal failure dogs supplemented with PUFA oils. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -1996 -THESIS -M36.