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dc.creatorNalim, F. Ameenaen_US
dc.date.accessioned2012-06-07T22:41:57Z
dc.date.available2012-06-07T22:41:57Z
dc.date.created1995en_US
dc.date.issued1995
dc.identifier.urihttp://hdl.handle.net/1969.1/ETD-TAMU-1995-THESIS-N35en_US
dc.descriptionDue to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to digital@library.tamu.edu, referencing the URI of the item.en_US
dc.descriptionIncludes bibliographical references.en_US
dc.descriptionIssued also on microfiche from Lange Micrographics.en_US
dc.description.abstractSclerotium rolfsii isolates from Texas peanut fields were grouped according to mycelial compatibility; 262 isolates arbitrarily-collected from plants showing symptoms of southern blight in four central Texas fields, and hyphal tipped, were examined for mycelial compatibility groups (MCG) based on the presence or absence of an antagonism zone (a clearing of mycelia) between paired colonies. These isolates could be placed in one of 12 MCG. MCG 6 was detected most frequently and was identified among isolates obtained from three widely separated fields. An additional seven MCG were identified in isolates collected from other locations in Texas. The internal transcribed spacer (ITS) region of the RDNA from several isolates belonging to different MCG was amplified by polymerase chain reaction (PCR) using the "Universal Primers" from ribosomal DNA. When amplification products of approximately 685 base pairs were digested with restriction endonuclease Mbo 1, four restriction digest patterns were observed. All isolates within an MCG consistently yielded the same restriction pattern and several MCG shared a common pattern. When a 18-base oligonucleotide primer, NK2, was used in PCR, three distinct amplification patterns were observed. Again, all isolates within a MCG had the same pattern and several MCG shared a common pattern.en_US
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.rightsThis thesis was part of a retrospective digitization project authorized by the Texas A&M University Libraries in 2008. Copyright remains vested with the author(s). It is the user's responsibility to secure permission from the copyright holder(s) for re-use of the work beyond the provision of Fair Use.en_US
dc.subjectplant pathology.en_US
dc.subjectMajor plant pathology.en_US
dc.titleA genetic study of mycelial compatibility groups of Sclerotium rolfsiien_US
dc.typeThesisen_US
thesis.degree.disciplineplant pathologyen_US
thesis.degree.nameM.S.en_US
thesis.degree.levelMastersen_US
dc.type.genrethesis
dc.type.materialtexten_US
dc.format.digitalOriginreformatted digitalen_US


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