STUDYING REGUATION OF NEU5AC (SIALIC ACID) BIOSYNTHESIS IN DROSOPHILA
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Glycoconjugates are very important compounds found in all domains of life. They are not primary products of genetic code like other structural biomolecules such as proteins and nucleic acids. They are product of glycosylation, i.e. addition of sugars to target molecules like proteins and lipids. Sialylated glycoconjugates are synthesized when sialic acids from donor molecules are transferred to the target molecules by sialyltransferase. These sialylated glycoconjugates are involved primarily in mediating the cell-cell interaction. Due to their prominent position on cell surface they act as a recognition site for various receptors as well as pathogens. Until now, most of the research about sialic acids and their functions is limited to deuterostomes. The information about the biosynthesis of sialic acid is incomplete and not well studied in protostomes. In Drosophila, the GNE enzyme that catalyzes the first two steps of sialic acid synthesis in vertebrates, is absent but the sialylated products can be detected in small quantities. I studied if an isozyme of GNE is present in Drosophila which catalyzes the rate limiting reaction of the glycosylation pathway. iv The results from immunoassay and western blot suggest that recombinant protein is being synthesized in the cytosol. At this moment, I am balancing the transgenic flies which will be tested for in vivo expression of GNE enzyme. I will perform physical tests to check for ectopic expression in these transgenic flies.
Pandey, Dheeraj (2011). STUDYING REGUATION OF NEU5AC (SIALIC ACID) BIOSYNTHESIS IN DROSOPHILA. Available electronically from