Show simple item record

dc.creatorHeare, Jake Emerson
dc.date.accessioned2011-08-04T14:30:50Z
dc.date.available2011-08-04T14:30:50Z
dc.date.issued2011-08-04
dc.identifier.urihttp://hdl.handle.net/1969.1/98368
dc.description.abstractHaplosporidium nelsoni (MSX) and Perkinsus marinus (Dermo) are protozoan parasites that are traditionally detected using time and labor intensive histological methods. Recently developed traditional PCR assays, specific for these parasites, were used to for initial screening of presence/absence in samples of the eastern oyster, Crassostrea virginica, collected from Galveston Bay, Aransas Bay, and Corpus Christi Bay, Texas. H. nelsoni (MSX) was not detected in any of the samples. P. marinus (dermo) was detected in oysters from all bays. Oysters that tested positive for P. marinus were further screened with quantitative PCR assays to enumerate the parasites. These data were directly compared to values obtained by Ray’s Fluid Thioglycollate histological method from the same sample. Though these tests have not been “ground-truthed” against the traditional histological methods it is the goal of this project to begin the process of comparing the two methods. There was strong agreement between the PCR and histological determination of P. marinus that is promising for eventual transition to PCR assays.en
dc.format.mediumelectronicen_US
dc.language.isoen_USen
dc.subjectRay's Fluid Thioglycollate Methoden
dc.subjectPerkinsus marinusen
dc.subjectHaplosporidium nelsonien
dc.subjectQuantitative real-time PCRen
dc.titleQUANTITATIVE REAL-TIME PCR DETECTION OF PERKINSUS MARINUS AND HAPLOSPORIDIUM NELSONI IN TEXAS OYSTERSen
dc.typeThesisen
dc.type.genreThesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record