QUANTITATIVE REAL-TIME PCR DETECTION OF PERKINSUS MARINUS AND HAPLOSPORIDIUM NELSONI IN TEXAS OYSTERS
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Haplosporidium nelsoni (MSX) and Perkinsus marinus (Dermo) are protozoan parasites that are traditionally detected using time and labor intensive histological methods. Recently developed traditional PCR assays, specific for these parasites, were used to for initial screening of presence/absence in samples of the eastern oyster, Crassostrea virginica, collected from Galveston Bay, Aransas Bay, and Corpus Christi Bay, Texas. H. nelsoni (MSX) was not detected in any of the samples. P. marinus (dermo) was detected in oysters from all bays. Oysters that tested positive for P. marinus were further screened with quantitative PCR assays to enumerate the parasites. These data were directly compared to values obtained by Ray’s Fluid Thioglycollate histological method from the same sample. Though these tests have not been “ground-truthed” against the traditional histological methods it is the goal of this project to begin the process of comparing the two methods. There was strong agreement between the PCR and histological determination of P. marinus that is promising for eventual transition to PCR assays.
SubjectRay's Fluid Thioglycollate Method
Quantitative real-time PCR
Heare, Jake Emerson (2011). QUANTITATIVE REAL-TIME PCR DETECTION OF PERKINSUS MARINUS AND HAPLOSPORIDIUM NELSONI IN TEXAS OYSTERS. Available electronically from