Changes in protein expression in maturing equine testis: a quantitative DIGE analysis
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Gross observation of testicular parenchyma of prepubertal horses reveals both light and dark regions. The light regions are associated with localized initiation and regulation of spermatogenesis. It is hypothesized that different proteins and protein expression levels in the light regions are factors contributing to successful sperm production. Differential Gel Electrophoresis (DIGE) and DeCyder Image analysis of testicular extracts from prepubertal stallions has identified forty proteins that may be involved in the regulation of the onset of spermatogenesis. Preliminary LC/MS/MS mass spectrometric analysis of six protein spots has been analyzed, but no protein identifications have yet been made. The results of this preliminary research are inconclusive with respect to protein identification, but the developments of protocols for procurement, protein extraction of light and dark tissue and DIGE analysis have been successful.
SubjectLC/MS/MS mass spectrometry
DeCyder Image Analysis
Roper-Foo, Pilar (2011). Changes in protein expression in maturing equine testis: a quantitative DIGE analysis. Available electronically from