In Vitro Culture of 'Dog Ridge' Grapevine
Vitis champini ‘Dog Ridge’ grapevine is a potentially desirable rootstock for Texas grapevines because of its disease resistance. This selection is reported to be difficult to root through hardwood cuttage as is often practiced for grapevine. A study was undertaken to establish a protocol to propagate ‘Dog Ridge’ grapevine in vitro by comparing combinations of explant type, basal salts, and benzyladenine (BA) concentration to proliferate shoots followed by in vitro and ex vitro rooting. Shoot tip and axillary bud explants were harvested from actively growing stock plants, disinfested with 10% v/v Clorox, rinsed in sterile distilled water and cultured on either Murashige and Skoog (MS) or Woody Plant Medium (WPM) containing 0, 4.4 and 8.8 μM BA for 12 weeks. Axillary bud explants cultured on MS medium proliferated better than shoot tips. Axillary bud explants cultured on media containing 4.4 or 8.8 μM BA proliferated better than shoot tip explants regardless of the BA concentration in the medium. Tissue cultured shoots rooted in either WPM medium without BA in vitro or in Redi-earth® potting mix ex vitro. Shoots developed roots in vitro better than under ex vitro conditions.
Wong, Kah-Yat Isaac (2009). In Vitro Culture of 'Dog Ridge' Grapevine. Available electronically from