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dc.contributor.advisorLineberger, R. Danielen_US
dc.creatorMaxwell, Douglas Wayneen_US
dc.date.accessioned2004-09-30T02:01:40Z
dc.date.available2004-09-30T02:01:40Z
dc.date.created2005-05en_US
dc.date.issued2004-09-30
dc.identifier.urihttp://hdl.handle.net/1969.1/454
dc.description.abstractResearch was undertaken to optimize propagation of the southern China native Camptotheca acuminata Decaisne, source of the medicinal compound camptothecin that is used in the treatment of multiple forms of cancer and other diseases. The study focused on cutting propagation, micropropagation, and seed storage. Softwood cuttings of C. acuminata rooted readily in intermittent mist (4 sec on every 6 min.) in coarse vermiculite when treated with K-IBA (indolebutyric acid, potassium salt) quick dips ranging from 4.14 mM to 37.3 mM, with a 29.0 mM quick dip (5 sec.) promoting 82% rooting with little foliar damage. Actively growing shoot tip explants were tissue cultured on media containing Murashige and Skoog, Gamborg's B5, and Woody Plant Medium (WPM) salts in factorial combinations with BA (benzyladenine). WPM containing 4.44 μM BA promoted excellent shoot proliferation; microcuttings were rooted, acclimated, and grown in the greenhouse. Seeds stored in polyethylene bags in a refrigerator (4°C) or freezer (-20°C) maintained good germination (81% and 80%, respectively) while seeds stored at room temperature (25°C) in polyethylene bags lost germination ability quickly (58%) after one year of storage. C. acuminata is readily adaptable to modern nursery techniques for either vegetative or seed propagation.en_US
dc.format.extent1508048 bytes
dc.format.extent74365 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectpropagationen_US
dc.subjectmedicinalen_US
dc.subjectcamptothecaen_US
dc.subjecttissue cultureen_US
dc.subjectseed storageen_US
dc.titlePropagation of Camptotheca acuminataen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentHorticultural Sciencesen_US
thesis.degree.disciplineHorticultureen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberWatson, W. Todden_US
dc.contributor.committeeMemberDavies, Fred T.en_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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