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    Transcriptional regulation and chromatin remodeling mechanisms at PHO5

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    Date
    2005-08-29
    Author
    Carvin, Christopher Dumas
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    Abstract
    Regulation of gene expression is vital for proper growth and prevention of disease states. In eukaryotes this regulation occurs in the context of chromatin which creates an inherent barrier for the binding of trans-acting factors, such as transcription factors and RNA polymerase. This dissertation focuses on the role of transcriptional activators and chromatin remodeling coactivators in the regulation of the repressible acid phosphatase gene PHO5. Our studies show that histone methylation at lysine 4 of histone H3 is required for the full repression of PHO5and GAL1-10. We show that bromodomains, a domain conserved in chromatin remodeling coactivators, may function to stabilize binding. Finally, we present a strategy using DNA methyltransferases as in vivo probes to detect DNA-protein interactions and examine chromatin structure. We extend this strategy to zinc-finger proteins which can be engineered to bind to any desired DNA sequence as a means of targeting methylation with potential use in epigenetic silencing.
    URI
    http://hdl.handle.net/1969.1/2193
    Subject
    chromatin
    Set1
    bromodomain
    PHO5
    targeted DNA methylation
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    • Electronic Theses, Dissertations, and Records of Study (2002– )
    Citation
    Carvin, Christopher Dumas (2003). Transcriptional regulation and chromatin remodeling mechanisms at PHO5. Doctoral dissertation, Texas A&M University. Texas A&M University. Available electronically from http : / /hdl .handle .net /1969 .1 /2193.

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