Effects of Nanostructured Materials on Bacteria
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Nanoparticles and nanostructured materials have attracted great interest for treating bacteria as a potential chemical-free method. This research carries out experiments to evaluate the antibacterial properties of nanoparticles and nanostructured materials. The commonly found bacterium Staphylococcus aureus was chosen for this study because of the extensive research and development surrounding the bacteria, its importance in human disease, and its extensive antibiotic resistance, particularly with methicillin-resistant S. aureus (MRSA) strains. The specific strain S. aureus Xen36 was selected due to its property of bioluminescence, which allows for real-time monitoring of the bacterial loads. Materials investigated were fabricated as particles and deposited on metallic substrates. Substrate materials of copper, aluminum, steel, and nickel were chosen because of their wide applications in manufacturing, particularly in the oil/gas industry. Results involving nanoparticles showed that Vv2Ov5 nanoparticles have potential antibacterial effects on S. aureus. They are effective in reducing the bacterial load after 2 and 24-hours of treatment. A decrease in bacterial load of 92.4%, 96.7%, and 94.3% was observed when cultured with the Vv2Ov5 nanoparticles at a concentration of 500ug/mL for 24 hours (NP concentration and incubation time), 1mg/mL for 2 hours, and 1mg/mL for 24 hours, respectively. Results involving nanostructured materials, i.e., nanoparticle Vv2Ov5 grown on nickel substrate. These materials cause a 99.1% decrease in bacterial load compared to a control over a 24-hour period.
Reed, Brady M (2018). Effects of Nanostructured Materials on Bacteria. Master's thesis, Texas A&M University. Available electronically from