Using an Ammonium Probe to Predict Ruminal Ammonia Concentration
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This study examined the possibility of developing a prediction equation for ruminal ammonia through comparing ruminal ammonia concentrations to ruminal ammonium levels. Ruminal ammonia concentrations were measured the standard way, using a catalyzed indophenol colorimetric reaction, and ammonium levels were measure using the HQ440D Laboratory Ammonium (NH4+) Ion Meter Package with ISENH4181 Ion Selective Electrode from HACH. In addition to these base measurements, the pH of each sample was measured to determine if the acid/base equilibrium was the driving factor behind the relationship. The ammonia concentration and ammonium concentrations were compared to determine what type of relationship there is between them in a ruminal environment. Since the ruminal ammonia levels are so low, 1-12 mmol, the pH does not appear to have any effect on the relationship. The initial data set showed a linear relationship between the two, but after running more trials, it was seen that a different equation was produced each time. The equations from all four trials had different slopes and different Y-intercepts. Without being able to determine a consistent relationship between ruminal ammonia and ammonium, a prediction equation was not able to be made.
Fitzgerald, Savannah G (2018). Using an Ammonium Probe to Predict Ruminal Ammonia Concentration. Undergraduate Research Scholars Program. Available electronically from