Show simple item record

dc.contributor.advisorKao, Katy C
dc.creatorPeabody V, George Lee
dc.date.accessioned2018-02-05T21:22:11Z
dc.date.created2017-08
dc.date.issued2017-07-24
dc.date.submittedAugust 2017
dc.identifier.urihttp://hdl.handle.net/1969.1/166071
dc.description.abstractAdaptive laboratory evolution facilitates the development and study of complex phenotypes. In an evolving population, individuals with mutations conveying a fitness benefit are selected for, and become enriched, in the environment. However, the rate of adaptation can be limited by the frequency of beneficial mutations; and competition amongst co-occurring beneficial mutations can lead to a loss of information. In this work, we describe the use of horizontal gene transfer (HGT) in conjunction with modulating mutation rate to more rapidly develop complex phenotypes in E. coli. We first characterize a previously developed “genderless” strain of E. coli proficient in continuous HGT during liquid culture. We next examine a few steps that can be taken to broaden and enhance the characteristics of this strain. We then introduced an inducible mutator system to the genderless strain in order allow modulation of mutation rate to enhance the supply of mutations during ALE. The strain was evolved in several well-characterized experimental environments to determine the influences of HGT and mutation rate on the rate of adaptation. The results indicate HGT and increasing mutation rate can act together to speed adaptive laboratory evolution, in many adaptive landscapes (environment). We then leveraged the HGT to more rapidly combine different complex phenotypes, to help expedite strain development of more industrially focused phenotypes. Finally, less developed works, which focus on applying different aspects of ALE toward strain development, are briefly discussed.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectEvolution
dc.subjectSexual Recombination
dc.titleSexual Recombination and the Development of Complex Phenotypes
dc.typeThesis
thesis.degree.departmentChemical Engineering
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorTexas A & M University
thesis.degree.nameDoctor of Philosophy
thesis.degree.levelDoctoral
dc.contributor.committeeMemberJayaraman, Arul
dc.contributor.committeeMemberWu, Hung-Jen
dc.contributor.committeeMemberManson, Michael
dc.type.materialtext
dc.date.updated2018-02-05T21:22:12Z
local.embargo.terms2019-08-01
local.embargo.lift2019-08-01
local.etdauthor.orcid0000-0001-6761-8575


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record