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dc.contributor.advisorGentry, Terry J
dc.creatorLaird, Edward Dylan
dc.date.accessioned2016-09-16T13:41:26Z
dc.date.available2016-09-16T13:41:26Z
dc.date.created2016-08
dc.date.issued2016-07-27
dc.date.submittedAugust 2016
dc.identifier.urihttps://hdl.handle.net/1969.1/157788
dc.description.abstractWastewater treatment plants (WWTP) are typically incapable of addressing the influx of antibiotics (AB), and may act as a harbor for the selection and proliferation of antibiotic resistant bacteria (ARB). In order to examine the influence of WWTP discharge on the AB resistance profiles of surface water bacteria in an urban stream setting, E. coli isolates and total heterotrophic bacteria populations were cultivated from 6 sampling sites up and downstream of WWTPs, and evaluated for resistance to selected ABs. Samples were collected over a 9-month period in the Carter’s Creek watershed of College Station, TX. E. coli isolates were tested for resistance to ampicillin, tetracycline, sulfamethoxazole, ciprofloxacin, cephalothin, cefoperazone, gentamycin, and imipenem using the Kirby-Bauer disc diffusion method. HPCs were cultivated on R2A amended with ampicillin, ciprofloxacin, tetracycline, and sulfamethoxazole. Significant associations (p < 0.05) were observed between the locations of sampling sites relative to WWTP discharge points and the rate of E. coli isolate resistance to tetracycline, ampicillin, cefoperazone, ciprofloxacin, and sulfamethoxazole; and an increased rate of isolate multi-drug resistance. The abundance of AB-resistant HPCs was significantly greater (p < 0.05) downstream of WWTPs for all treatments; however, there was no spatially significant difference when normalized to total HPCs cultivated with no AB. Results suggest that the effects of human development, specifically the discharge of treated WWTP effluent into surface waters, are potentially significant contributors to the spread and persistence of AB resistance in the surrounding watershed.en
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectantibiotic resistanceen
dc.subjectsurface wateren
dc.subjectwastewater treatment plantsen
dc.subjectbacteriaen
dc.subjectenvironmentalen
dc.titleCharacterization of Antibiotic Resistance Profiles of Surface Water Bacteria in an Urbanizing Watersheden
dc.typeThesisen
thesis.degree.departmentSoil and Crop Sciencesen
thesis.degree.disciplineWater Management and Hydrological Scienceen
thesis.degree.grantorTexas A & M Universityen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
dc.contributor.committeeMemberBrooks, John
dc.contributor.committeeMemberKarthikeyan, Raghupathy
dc.contributor.committeeMemberMarcantonio, Franco
dc.type.materialtexten
dc.date.updated2016-09-16T13:41:27Z
local.etdauthor.orcid0000-0002-2414-5955


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