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Localization of the Osseous Boundaries of the Regional Acceleratory Phenomenon
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The purpose of this study was to evaluate the amount of damage induced by micro-osteoperforations (MOPs) on the cortical and trabecular bone, as well as to quantify the extent of the subsequent remodeling that occurs in and around the injury site. Using a split mouth design, 34 MOPs (PROPEL) were randomly placed in the mandibular furcal bone of 13 beagle dogs, either 2 or 4 weeks prior to sacrifice. The control side received no treatment. µCT, Vickers Hardness microindentation, histologic and histomorphometric analyses were performed. Microfractures extended up to approximately 0.8 mm. The density of the experimental cortical and trabecular bone was significantly less than control bone up to 4.2 mm from the MOP margin. However, density differences between the experimental and control samples were less than 5% beyond 1.5 mm from the MOP edge. The Vickers Hardness of the experimental cortical bone was significantly less than the control bone up to 0.75 mm from the MOP edge after two weeks of healing and 0.5 mm after four weeks of healing. TRAP staining showed a dramatic increase in TRAP activity up to approximately 2.5 mm from the MOP margin at two weeks. However by four weeks, the TRAP activity had diminished to control levels. Vital fluorescence staining showed diffuse bone deposition up to 1.5 mm from the MOP margin, as early as 2 weeks after MOP placement. Beyond 1.0-1.5 mm, fluorescence labeling shows activity levels were similar to control levels at both 2 and 4 weeks. H&E sections of cortical and trabecular bone after 2 weeks showed small areas of woven bone within the MOP site, as well as an area of acellular bone extending approximately 0.5 mm. After four weeks of healing, there were greater amounts of woven bone, as well as early signs of lamellar bone, in and around the MOP site. In conclusion, the demineralization associated with the placement of micro-osteoperforations is transient and healing of the injured area, as well as remineralization of bone affected by MOP placement begins as early as one week after the trauma. While the biologic effects of MOP placement extend further, the principal effects are limited to approximately 1.5 mm.
Van Gemert, Lauren Nicole (2016). Localization of the Osseous Boundaries of the Regional Acceleratory Phenomenon. Master's thesis, Texas A & M University. Available electronically from