The Ability of a Cell Salvage System and Leukocyte Reduction Filter to Remove Hemangiosarcoma Cells from Dog Blood
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Hemangiosarcoma is the neoplastic growth of primitive cells that resemble endothelial cells. Dogs with hemangiosarcoma often present on an emergency basis due to tumor rupture and intraabdominal bleeding. Treatment involves hemodynamic stabilization, surgery to remove the bleeding tumor and chemotherapy to address micrometastatic disease, which is presumed to be present at the time of diagnosis. Allogeneic blood transfusions are often required in the perioperative period to address blood loss. With recent advances in human medicine, the liberal use of allogeneic blood products in patients with cancer has been called into question. Intraoperative cell salvage offers an alternative method to restore blood volume in dogs with intraabdominal bleeding, but the concern for iatrogenic tumor cell dissemination exists. The first objective of this study was to determine if the administration of allogeneic blood products negatively affects time to disease progression in dogs undergoing surgery for hemangiosarcoma. The second objective of this study was to determine the ability of an intraoperative cell salvage system with a leukocyte reduction filter to remove hemangiosarcoma cells from dog blood ex vivo. Retrospective data were collected from two hospitals to include dogs undergoing surgery for hemangiosarcoma. Six variables were analyzed to determine predictors of time to disease progression. The administration of allogeneic blood products was found to be independently associated with shorter time to disease progression. For the second objective, cultured hemangiosarcoma cells were added to dog blood to represent the blood that may be encountered during surgery to address a bleeding tumor. The solution was processed through the intraoperative cell salvage machine followed by a leukocyte reduction filter. PCR, flow cytometry and cytologic examination were used to determine the presence of cultured hemangiosarcoma cells at different points in the intraoperative cell salvage system processing. The processing removed a majority hemangiosarcoma cells from dog blood.
intraoperative cell salvage
leukocyte reduction filter
transfusion related immunomodulation
Ciepluch, Brittany Jane (2016). The Ability of a Cell Salvage System and Leukocyte Reduction Filter to Remove Hemangiosarcoma Cells from Dog Blood. Master's thesis, Texas A & M University. Available electronically from