Effects of Estrogen and Phytoestrogens on the Development of Colonic Inflammation
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Colon cancer exhibits the third highest cancer mortality rate of the US. Patients with inflammatory bowel disease (IBD) display a much higher risk for development of the disease. Women experience protective effects against colon cancer due to naturally increased levels of estrogen compared to men. Preliminary studies were performed to determine methods of producing an IBD mouse model displaying suitable levels of inflammation for future studies regarding the effects of estrogen in a precancerous state. By inducing colon inflammation to create an IBD model, appropriate levels of estrogen supplementation will be obtained to enhance the results of future investigations. In phase one, male mice were exposed intrarectally to varying amounts of trinitrobenzene sulfonic acid (TNBS), and a relevant IBD state was achieved by the 3% TNBS dose, which was then used in phase two. For this phase, colitis was induced with TNBS in ovariectomized (OVX) female mice supplemented with varied levels of estrogen. TNBS induced inflammation and was not detrimental to mice at the 3% TNBS solution exposure level. OVX mice supplemented with estrogen achieved the colitis state without additional health implications. Mice supplemented with .5 mg implants displayed estrogen blood levels half the value of those with 1 mg estrogen implants. Results obtained in this pilot study represent the first step toward determining how estrogen may decrease the risk of inflammation-associated development of colon cancer.
Galipp, Kari (2012). Effects of Estrogen and Phytoestrogens on the Development of Colonic Inflammation. Honors and Undergraduate Research. Available electronically from