The Role of miR-143 and miR-145 in the Invasion of Glioblastoma
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Glioblastoma multiforme (GBM) is the most common and most malignant type of primary brain tumor. It is highly invasive and therefore difficult to treat. The life expectancy of patients harboring GBM is around 12-18 months, even in the best clinical trials. GBM invasion prevents a surgical cure; by the time the diagnosis is made, tumor cells have invaded normal tissue remote from the tumor mass. Small noncoding RNAs may contribute to the invasive phenotype of GBM. MicroRNAs (miRNAs) are small, single stranded noncoding regulatory RNA molecules that function to modulate the activity of specific mRNA targets and play important roles in a wide range of physiological and pathological processes. A previously established in vitro method was used to create GBM sub-populations with enhanced invasion (IM3 subpopulations). Comparison of micro-RNA expression profiles between GBM parental cell lines and IM3 sub-populations revealed differentially expressed miRNAs between the two cell lines. Two of these miRNAs, miR-143 and mIR-145 were found to be largely overexpressed in the IM3 subpopulations and may serve as potential mediators of the invasive phenotype. Knockdown of these miRNAs in U87 cell lines showed an alteration in GBM invasion. These miRNAs may serve as therapeutic targets that decrease tumor invasion.
Ronck, Matthew K 1984- (2010). The Role of miR-143 and miR-145 in the Invasion of Glioblastoma. Honors and Undergraduate Research. Available electronically from