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dc.contributor.advisorCriscione, Johnen_US
dc.creatorJetton, Emily Hopeen_US
dc.date.accessioned2004-11-15T19:50:27Z
dc.date.available2004-11-15T19:50:27Z
dc.date.created2004-08en_US
dc.date.issued2004-11-15
dc.identifier.urihttp://hdl.handle.net/1969.1/1216
dc.description.abstractIn order to understand the mechanical functions of the cardiac muscle it is important to first understand the microstructure of the tissue. Young et al. (1998) realized that quantitative three-dimensional information about the ventricular myocardium is necessary to analyze myocardial mechanics. They developed a technique using confocal fluorescence laser scanning microscopy to obtain three-dimensional images. While this method worked well in rebuilding the myocardial tissue image by image, it was quite extensive and costly. Costa et al. (1999) developed a method that was used to perform three-dimensional reconstruction as well. Their method, while less expensive and much less time consuming, required sheet assumptions and did not look directly at the cross-fiber plane. From Dr. Criscione's previous work on canines (Ashikaga et al., 2004), we found that the sheet structure can be accurately determined from cross-fiber sections without making any sheet assumptions. We have now expanded on those ideas and created a method to perform the quantitative histological investigation of the rat hearts in a way that is both timely and cost effective. We developed a processing method that preserves the orientation of the fiber and sheet angles. This method was carried out using plastic embedding since the dehydration process used in paraffin embedding has a tendency to grossly distort tissue. Once the heart was fixed in formalin, we then removed the septum and sliced it several times vertically. This allowed us to image the tissue at several depths and find an average fiber angle for each slice. Next, the specimen was hardened, and the sheet orientation was evaluated using polarized light. Once both fiber and sheet angles were obtained from several depths within the septum, we then constructed a three-dimension model of the wall. This method was both cost effective and less time consuming than previous ones and will be a method that can be used in the future to compare the myocardial tissue of diseased and healthy rat hearts so that we may better understand the mechanical functions of the heart as it remodels due to disease.en_US
dc.format.extent1063528 bytes
dc.format.extent77643 bytes
dc.format.mediumelectronicen_US
dc.format.mimetypeapplication/pdf
dc.format.mimetypetext/plain
dc.language.isoen_USen_US
dc.publisherTexas A&M Universityen_US
dc.subjectquantitativeen_US
dc.subjecthistologyen_US
dc.subjectmicrostructureen_US
dc.subjecthearten_US
dc.titleThe development of processing methods for a quantitative histological investigation of rat heartsen_US
dc.typeBooken
dc.typeThesisen
thesis.degree.departmentBiomedical Engineeringen_US
thesis.degree.disciplineBiomedical Engineeringen_US
thesis.degree.grantorTexas A&M Universityen_US
thesis.degree.nameMaster of Scienceen_US
thesis.degree.levelMastersen_US
dc.contributor.committeeMemberQuick, Christopheren_US
dc.contributor.committeeMemberHumphrey, Jayen_US
dc.type.genreElectronic Thesisen_US
dc.type.materialtexten_US
dc.format.digitalOriginborn digitalen_US


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