Abstract
Arcobacter butzleri is a pathogenic bacterium that has been found in dairy cattle, pigs, poultry and humans. As of this writing, there are no data to report the prevalence of occurrence of A. butzleri in beef cattle. Also, there are numerous culture methods used to isolate this organism and little data comparing their efficacies. The objectives of this study were to determine the prevalence of occurrence of A. butzleri in cattle from Texas and compare the effectiveness of two isolation methods for this purpose. The two isolation methods compared were the Johnson-Murano method and the Collins method. Cattle (n = 200) from two feedlots, a dairy, and a stocker were sampled. Fecal swabs were obtained from cattle and each sample was cultured using four methods. Two of those methods were the JM method and the Collins method and the other two were combinations of the previously mentioned methods. Identification of the isolates to confirm A. butzleri was done using Polymerase Chain Reaction. No significant differences were found between culture methods. Of the cattle sampled using all media results, 18 of 200 tested positive for A. butzleri. The JM method detected this organism in 9 of 200 samples. The Collins method detected this organism in 5 of 200 samples.
Golla, Steven Craig (2000). Determination of the occurrence of Arcobacter butzleri in beef and dairy cattle from Texas using two isolation methods. Master's thesis, Texas A&M University. Available electronically from
https : / /hdl .handle .net /1969 .1 /ETD -TAMU -2000 -THESIS -G66.